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Agarose Electrophoresis Skin gels Preparation and Practice

AGAROSE ELECTROPHORESIS GEL PREPARATION AND PRACTICE SAMPLE LAUNCHING STANDARD OPRERATING PROCEDURE

ID NUMBER 1PREPARDED BY CHRIS FOLEY

MODIFICATION NUMBER 1PAGE 1 OF 3

DATE OF REVISION 2-1-12

1 . ASSERTION OF GOAL

Agarose Electrophoresis gel practice and preparation.

2 . OPPORTUNITY

Prepare a SOP for the preparation of 1% agarose gel employing TBE barrier and practice electrophoresis solution loading treatment.

3. DECLARATION OF RESPONSIBILITY

This procedure have been carried out by Frank Foley

5. MATERIALS NEEDED

Calculatorweighting vessels

Scale1000ml beaker

Spatula500ml graduated cylinder

Magnet stirrerstir club

Hot plateparafilm

Electrophoresis chambergel bed with well previous

Micropipette tipssample loading solution

Distilled waterlabeling tape

Gogglesgloves

250 cubic centimeters glass flaskthermometer

a few. REAGENTS AND CHEMICALS

A. Tris BaseManufacturerFisher Scientific

Whole lot Number976707A

Formulation Weight121. 16 grams

M. Boric AcidManufacturerFisher Scientific

Great deal Number99715

Molecular Weight61. 83grams

SOP AGROSE GEL ELECTROPHORESIS PREP & PRACTICEPAGE two OF 22-1-12 C. EDTAManufacturerFisher Scientific

Great deal Number 073458

Formula Weight372. 24grams

Deb. AgaroseEdvotek

Whole lot LF45100003

6. CALCULATIONS

Computations for organizing the grams needed to produce 500ml of Trisborate barrier using concentrations of reactants listed below. Compute grams of solute multiply ml time's mole time's molecular weight. A. zero. 89mM Collections Base

zero. 00089m times. 500l back button 121. 14g/m = 0. 053 grams needed

N. 0. 89mM Boric Acid solution

0. 00089m x. 500l x sixty one. 83g/m = 0. 027 grams needed

C. 2 . 5mM Na2EDTA

. 0025m times. 500l times 372. 24g/m = zero. 465 grms needed

several. PROCEDURE

A. Buffer Preparation

1 . Place 1000ml beaker onto blend plate containing 60ml of distilled water and permanent magnetic stirrer, start stir dish 2 . Put above worked out...

References: 1 ) Klein, Jessie. " Preparing of an Agrarose Electrophoresis Skin gels and Practice Sample Loading” Middlesex Community College, 11/20/1996.

01.09.2019

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